Comparison of quantitative real-time PCR and short-term (18-hour) microculture in diagnosis of fetal cytomegalovirus infection: Impact of hyperimmunoglobulin treatment
Lukas Penka
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tübingen, Germany
Search for more papers by this authorKarl-Oliver Kagan
Department of Obstetrics and Gynaecology, University Hospital of Tuebingen, Tübingen, Germany
Search for more papers by this authorRangmar Goelz
Department of Neonatology, University Children's Hospital Tuebingen, Tuebingen, Germany
Search for more papers by this authorCorresponding Author
Klaus Hamprecht
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tübingen, Germany
Correspondence
Klaus Hamprecht, Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Elfriede-Aulhorn-Straße 6, 72076, Tuebingen, Germany.
Email: [email protected],
Search for more papers by this authorLukas Penka
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tübingen, Germany
Search for more papers by this authorKarl-Oliver Kagan
Department of Obstetrics and Gynaecology, University Hospital of Tuebingen, Tübingen, Germany
Search for more papers by this authorRangmar Goelz
Department of Neonatology, University Children's Hospital Tuebingen, Tuebingen, Germany
Search for more papers by this authorCorresponding Author
Klaus Hamprecht
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tübingen, Germany
Correspondence
Klaus Hamprecht, Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Elfriede-Aulhorn-Straße 6, 72076, Tuebingen, Germany.
Email: [email protected],
Search for more papers by this authorAbstract
Objective
The prognostic value of human cytomegalovirus detection (HCMV) DNA levels from amniotic fluid (AF) for the outcome of the infected newborn is still a matter of debate, especially if the onset of maternal primary infection at amniocentesis is unknown. The objective of this study was to investigate the analytical performance in short-term (18-hour) microculture from preconcentrated samples and quantitative real-time PCR (rtPCR) for diagnosis of fetal HCMV infection.
Methods
A retrospective diagnostic study was conducted on 51 AF samples taken from women that transmitted HCMV prenatally. Amniocentesis was performed around 22-week gestation. The samples were tested for HCMV viral load via quantitative rtPCR and additionally with quantitative short-term (18-hour) microculture following preconcentration via a 50 000 g centrifugation step prior to inoculation to fibroblast monolayers.
Results
Both methods show correlating results (ρ = 0.903). In 25 samples, the women received intravenous hyperimmunoglobulin prior to amniocentesis resulting in a lower correlation of both quantitative methods (ρ = 0.445), in reduced median copy numbers of HCMV DNA (P = .037) and reduced viral infectivity in short-term microculture (P = .025).
Conclusion
Both methods lead to correlating results using AF samples from HIG-naïve women. Human cytomegalovirus viral load and infectivity in cell culture are reduced in samples following maternal hyperimmunoglobulin treatment.
CONFLICTS OF INTEREST
None declared
Supporting Information
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